The Light Sheet instrument at the University of Michigan Microscopy Core provides researchers with advanced tools to study dynamic subcellular processes in living cells. Supported by the Biosciences Initiative (BSI), the core features the Zeiss Lattice Light Sheet 7, a cutting-edge instrument designed for long-term, gentle imaging of live cells.
Mission and Goals
The Lattice Light Sheet enables high-quality live cell imaging for researchers at U-M and beyond. By offering state-of-the-art technology and expert support, the microscopy core aims to facilitate discoveries in cell biology, cancer research, developmental biology, and more.
Facilities and Equipment
The core is equipped with the Zeiss Lattice Light Sheet 7, which offers:
- Fast and Gentle Imaging: Allows volumetric imaging with minimal photobleaching and phototoxicity.
- Long-term Imaging: Ideal for observing dynamic subcellular processes over hours or days.
- Specifics: 40X objective and three laser lines (488nm, 561nm, 633nm), suited for samples up to 50μm thick.
Services Offered
The Light Sheet Core provides a range of services, including:
- High-Resolution Imaging: Using the Zeiss Lattice Light Sheet 7 for detailed observation of live cells.
- Training and Support: Comprehensive training sessions and ongoing support from highly skilled staff, including PhD-level experts.
- Project Consultation: Assistance with experimental design, sample preparation, and image analysis
Collaborating with the Core
Researchers can access the Light Sheet Core’s resources through the following steps:
- Getting Started: Review the Getting Started guide and prepare the necessary samples.
- Complete a Request Form: Submit the form to request services or instrument time.
- Consultation: Schedule a meeting to discuss project needs and strategies with core staff, who provide guidance from experimental design to publication.
Prospective users can email Jennifer Peters, Ph.D., the managing director for the microscopy core, at jenpete@umich.edu.
Conclusion
The Light Sheet Core at U-M is a vital resource for live cell imaging, providing essential tools and expertise to drive innovation. By offering access to the Zeiss Lattice Light Sheet 7 and comprehensive support services, the core empowers researchers to achieve groundbreaking discoveries. Looking forward, the core aims to continue enhancing its capabilities and expanding its impact through ongoing innovation and collaboration.
For more detailed information, visit the Microscopy Core webpage.
